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(1996) cloned a human C-C chemokine receptor gene from a human genomic DNA library based on its similarity to a murine C-C chemokine receptor clone (MOP020).
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With a set of overlapping lambda clones, they showed that the gene is 17.5 kb from the CMKBR2 gene (CCR2; 601267).
The 2 coding regions share 75% DNA and amino acid sequence identity. (1997) analyzed the genomic structure of CCR5, which contains 4 exons, spanning approximately 6 kb, and only 2 introns. Exon 4 contains the open reading frame, the complete 3-prime UTR, and 11 nucleotides of the 5-prime UTR.
The regulatory sequences and noncoding exons are polymorphic, whereas the protein sequence is not. (1998) showed that polarized Th1 cells preferentially express CXCR3 (300574) and CCR5.
In contrast, Th2 cells preferentially express CCR4 (604836) and, at least in a subpopulation of Th2 cells, CCR3 (601268). (1996) functionally expressed the CCR5 gene in a stably transfected CHO-K1 cell line.Furthermore, and remarkably, 14 subjects remained unaffected to the time of the report, and in these subjects homozygous CCR5 mutations were found in none, but in most of them there was overproduction of beta-chemokines.In vitro experiments confirmed the potent anti-HIV suppressive effect of these chemokines.In transfected cells, macrophage inflammatory protein (MIP)-1-alpha (182283) appeared to be the most potent agonist for CCCKR5, with MIP-1-beta (CCL4; 182284) and RANTES (CCL5; 187011) also active at physiologic concentrations. (1996) detected transcript from the gene in a promyeloblastic cell line, which suggested a potential role for the chemokine receptor in granulocyte lineage proliferation and differentiation. (1996) showed that MIP-1-alpha, MIP-1-beta, and RANTES each inhibit infection of CD4 cells by primary, nonsyncytium-inducing (NSI) HIV-1 strains at the virus entry stage and also block env-mediated cell-cell fusion.Both groups showed that expression of the CCCKR5 protein renders nonpermissive CD4 cells susceptible to infection by HIV-1 strains (see 609423).亚洲人和西方人的社交网络 • アジアと西洋の社会的ネットワーク • 아시아와 서양인의 소셜 네트워크 The AMWF Social Network is a virtual community for Asian guys and White girls, Black girls, Hispanic girls, etc. Lots of cute and hot asian guys and girls on the site.Tags: Adult Dating, affair dating, sex dating